RPL17 Polyclonal antibody
RPL17 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14121-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse pancreas tissue, rat pancreas tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
14121-1-AP targets RPL17 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPL17 fusion protein Ag5270 |
| Full Name | ribosomal protein L17 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC066323 |
| Gene Symbol | RPL17 |
| Gene ID (NCBI) | 6139 |
| RRID | AB_2253985 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL17 antibody 14121-1-AP | Download protocol |
| IHC protocol for RPL17 antibody 14121-1-AP | Download protocol |
| IP protocol for RPL17 antibody 14121-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Identifying the functional contribution of the defatty-acylase activity of SIRT6. | ||
Elife METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance. | ||
Front Endocrinol (Lausanne) GIT2 Acts as a Systems-Level Coordinator of Neurometabolic Activity and Pathophysiological Aging. | ||
J Pineal Res Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin |
