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RPP21 Polyclonal antibody

RPP21 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 16377-1-AP

Synonyms

C6orf135, CAT60, Ribonucleoprotein V, RNaseP protein p21, RPP21

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using RPP21 Polyclonal antibody (16377-1-AP)

    WB analysis of COLO 320 using 16377-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using RPP21 Polyclonal antibody (16377-1-AP)

    WB analysis of COLO 320 using 16377-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of COLO 320 cells using RPP21 Polyclonal antibody (16377-1-AP)

    WB analysis of COLO 320 using 16377-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using RPP21 Polyclonal antibody (16377-1-AP)

    WB analysis of COLO 320 using 16377-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16377-1-AP (RPP21 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16377-1-AP targets RPP21 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPP21 fusion protein Ag9353
Full Name ribonuclease P/MRP 21kDa subunit
Calculated Molecular Weight 154 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC011730
Gene Symbol RPP21
Gene ID (NCBI) 79897
RRIDAB_2878250
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPP21 antibody 16377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols