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RPRD1B Polyclonal antibody

RPRD1B Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24542-1-AP

Synonyms

C20orf77, CREPT, RPRD1B

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RPRD1B Polyclonal antibody (24542-1-AP)

    WB analysis of HeLa using 24542-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RPRD1B Polyclonal antibody (24542-1-AP)

    WB analysis of Jurkat using 24542-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24542-1-AP (RPRD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPRD1B Polyclonal antibody (24542-1-AP)

    IF Staining of HeLa using 24542-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24542-1-AP (RPRD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24542-1-AP is RPRD1B Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPRD1B fusion protein Ag21578
Full Name regulation of nuclear pre-mRNA domain containing 1B
Calculated Molecular Weight 326 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC033629
Gene Symbol RPRD1B
Gene ID (NCBI) 58490
RRIDAB_2879597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPRD1B antibody 24542-1-APDownload protocol
IF protocol for RPRD1B antibody 24542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PINK1-deficient Human Dopamine Neurons

Authors - Hu Wang
  • KO Validated
View Article