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RRAS Polyclonal antibody

RRAS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27457-1-AP

Synonyms

p23, Ras related protein R Ras, RRAS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RRAS Polyclonal antibody (27457-1-AP)

    WB analysis using 27457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RRAS Polyclonal antibody (27457-1-AP)

    WB analysis of A549 using 27457-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RRAS Polyclonal antibody (27457-1-AP)

    WB analysis of PC-3 using 27457-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 27457-1-AP (RRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HT-29 cells using RRAS Polyclonal antibody (27457-1-AP)

    IP experiment of HT-29 using 27457-1-AP

    IP result of anti-RRAS (IP:27457-1-AP, 4ug; Detection:27457-1-AP 1:500) with HT-29 cells lysate 3320 ug.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of human renal cell carcinoma using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of human renal cell carcinoma using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of mouse kidney using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of mouse colon using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of mouse colon using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of human gliomas using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of human gliomas using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RRAS Polyclonal antibody (27457-1-AP)

    IHC staining of mouse kidney using 27457-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27457-1-AP (RRAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, PC-3 cells, HT-29 cells
Positive IP detected inHT-29 cells
Positive IHC detected inhuman renal cell carcinoma tissue, mouse kidney tissue, mouse colon tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

27457-1-AP targets RRAS in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRAS fusion protein Ag26538
Full Name related RAS viral (r-ras) oncogene homolog
Calculated Molecular Weight 218 aa, 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC016318
Gene Symbol RRAS
Gene ID (NCBI) 6237
RRIDAB_2880876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RRAS antibody 27457-1-APDownload protocol
IHC protocol for RRAS antibody 27457-1-APDownload protocol
IP protocol for RRAS antibody 27457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human，mouseWB

Biomaterials

Polymeric nanoparticle mediated inhibition of miR-21 with enhanced miR-124 expression for combinatorial glioblastoma therapy.

Authors - Yuanyuan Liu
View Article
humanWB

Transl Cancer Res

Identification of RRAS gene related to nasopharyngeal carcinoma based on pathway and network-based analyses.

Authors - Ruowen Xiao
View Article
humanWB

Hepatology

Metabolism-induced tumor activator 1 (MITA1), an Energy Stress-Inducible Long Noncoding RNA, Promotes Hepatocellular Carcinoma Metastasis

Authors - Meilin Ma
View Article