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RRM1-Specific Polyclonal antibody

RRM1-Specific Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19686-1-AP

Synonyms

R1, ribonucleotide reductase M1, RIR1, RR1, RRM1, RRM1-Specific

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using RRM1-Specific Polyclonal antibody (19686-1-AP)

    WB analysis of A549 using 19686-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RRM1-Specific Polyclonal antibody (19686-1-AP)

    WB analysis of HeLa using 19686-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RRM1-Specific Polyclonal antibody (19686-1-AP)

    WB analysis of HEK-293 using 19686-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19686-1-AP (RRM1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RRM1-Specific Polyclonal antibody (19686-1-AP)

    IF Staining of HepG2 using 19686-1-AP

    Immunofluorescent analysis of HepG2 cells, using RRM1 antibody 19686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19686-1-AP targets RRM1-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ribonucleotide reductase M1
Calculated Molecular Weight 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberNM_001033
Gene Symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_10697654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RRM1, also named as RR1, belongs to the ribonucleoside diphosphate reductase large chain family. RRM1 provides the precursors necessary for DNA synthesis. RRM1 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.RRM1 is a key enzyme in DNA synthesis: low level expression of RRM1 is associated with poor survival. RRM1 is a prognostic marker of NSCLC. The antibody is specfic to RRM1.

Protocols

Product Specific Protocols
WB protocol for RRM1-Specific antibody 19686-1-APDownload protocol
IF protocol for RRM1-Specific antibody 19686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols