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RSPO3 Monoclonal antibody

RSPO3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E4F1

Cat no : 66314-1-Ig

Synonyms

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    WB analysis of human skeletal muscle using 66314-1-Ig

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RSPO3 Monoclonal antibody (66314-1-Ig)

    WB analysis of A549 using 66314-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    IHC staining of human lung cancer using 66314-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RSPO3 Monoclonal antibody (66314-1-Ig)

    IHC staining of human lung cancer using 66314-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66314-1-Ig (RSPO3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RSPO3 Monoclonal antibody (66314-1-Ig)

    IF Staining of A549 using 66314-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 66314-1-Ig(RSPO3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue, A549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66314-1-Ig targets RSPO3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RSPO3 fusion protein Ag9899
Full Name R-spondin 3 homolog (Xenopus laevis)
Calculated Molecular Weight 272 aa, 31 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC022367
Gene Symbol RSPO3
Gene ID (NCBI) 84870
RRIDAB_2881695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

Protocols

Product Specific Protocols
WB protocol for RSPO3 antibody 66314-1-IgDownload protocol
IHC protocol for RSPO3 antibody 66314-1-IgDownload protocol
IF protocol for RSPO3 antibody 66314-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols