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RUVBL2 Monoclonal antibody

RUVBL2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 67851-1-Ig

Synonyms

48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUVBL2 Monoclonal antibody (67851-1-Ig)

    WB analysis using 67851-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67851-1-Ig (RUVBL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RUVBL2 Monoclonal antibody (67851-1-Ig)

    IHC staining of human lung cancer using 67851-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67851-1-Ig (RUVBL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RUVBL2 Monoclonal antibody (67851-1-Ig)

    IHC staining of human lung cancer using 67851-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67851-1-Ig (RUVBL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RUVBL2 Monoclonal antibody (67851-1-Ig)

    IF Staining of HeLa using 67851-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RUVBL2 antibody (67851-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA549 cells, A431 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67851-1-Ig targets RUVBL2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RUVBL2 fusion protein Ag0253
Full Name RuvB-like 2 (E. coli)
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC000428
Gene Symbol RUVBL2
Gene ID (NCBI) 10856
RRIDAB_2918610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUVBL2 belongs to the conserved ATPases associated with various cellular activities (AAA+) protein subfamily, which is characterized by the presence of conserved Walker A and B motifs that are involved in ATP binding and hydrolysis. The AAA+ (ATPases associated with diverse cellular activities) ATPases, RUVBL1 and RUVBL2, were originally isolated as components of transcriptional complexes and shown to function in a number of different cellular processes, including transcriptional regulation, chromatin remodelling and DNA damage responses. (PMID: 31572066, PMID: 31138842)

Protocols

Product Specific Protocols
WB protocol for RUVBL2 antibody 67851-1-IgDownload protocol
IHC protocol for RUVBL2 antibody 67851-1-IgDownload protocol
IF protocol for RUVBL2 antibody 67851-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols