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Rad51D Polyclonal antibody

Rad51D Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11126-1-AP

Synonyms

HsTRAD, R51H3, RAD51 homolog D, RAD51 like 3 (S. cerevisiae), RAD51 like protein 3, Rad51D, RAD51L3, Trad

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Rad51D Polyclonal antibody (11126-1-AP)

    WB analysis using 11126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11126-1-AP (Rad51D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using Rad51D Polyclonal antibody (11126-1-AP)

    IHC staining of human lymphoma using 11126-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11126-1-AP (Rad51D antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, A2780 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11126-1-AP targets Rad51D in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Rad51D fusion protein Ag1595
Full Name RAD51-like 3 (S. cerevisiae)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC014422
Gene Symbol RAD51L3
Gene ID (NCBI) 5892
RRIDAB_10644480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAD51L3, also named RAD51D, R51H3 and TRAD, belongs to the recA family and RAD51 subfamily. It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA, and specifically to nicks in duplex DNA. The Heterodimer of RAD51L3 is about 70 kDa.

Protocols

Product Specific Protocols
WB protocol for Rad51D antibody 11126-1-APDownload protocol
IHC protocol for Rad51D antibody 11126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols