Rat IL-2 ELISA Kit

Sensitivity

8.3 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Rat

Cat no : KE20002

Synonyms

Il2, Il-2, interleukin 2



Product Information

KE20002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL2. The assay recognizes mouse IL2. An antibody specific for IL2 has been pre-coated onto the microwells. The IL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL2 is added to detect the captured IL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Rat IL-2 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 8.3 pg/mL
Range 31.25-2000 pg/mL
Reactivity Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 116562

Recovery

Sample TypeAverageRange
Cell culture supernatants106%93%-119%
Rat Serum97%83%-116%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12038.42.46.2
220219.49.84.5
3201,013.162.16.1

InterAssay

Samplenmean ( pg/mL)SDCV%
12437.02.36.2
224225.517.27.6
324996.884.68.5

Background Information

Interleukin-2 (IL2) is a protein initially identified as a T cell growth factor. IL2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that IL2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (IL2R). IL2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il2, Il-2, interleukin 2

Publications

SpeciesSample TypeTitle
ratplasma

Evid Based Complement Alternat Med

Anti-inflammatory Mechanism of Ruzu Bitters on Diet-Induced Nonalcoholic Fatty Liver Disease in Male Wistar Rats.

Authors - Olubanke O Ogunlana