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  • Featured Product
  • KD/KO Validated

S100A1 Polyclonal antibody

S100A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16027-1-AP

Synonyms

Protein S100 A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100, S100 alpha, S100A, S100A1

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using S100A1 Polyclonal antibody (16027-1-AP)

    WB analysis of human heart using 16027-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using S100A1 Polyclonal antibody (16027-1-AP)

    WB analysis of human heart using 16027-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 16027-1-AP (S100A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human malignant melanoma using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human malignant melanoma using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of rat brain tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of rat brain using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of rat brain using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human thyroid cancer using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human thyroid cancer using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human tonsillitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human kidney using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human kidney using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16027-1-AP (S100A1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human ovary tumor using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human ovary tumor using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IHC staining of human appendicitis using 16027-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16027-1-AP (S100A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human ovary cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IF Staining of human ovary cancer using 16027-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human ovary cancer tissue using S100A1 Polyclonal antibody (16027-1-AP)

    IF Staining of human ovary cancer using 16027-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human ovary cancer cells using 16027-1-AP (S100A1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman malignant melanoma tissue, human kidney tissue, human ovary tumor tissue, human appendicitis tissue, human tonsillitis tissue, rat brain tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman ovary cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16027-1-AP targets S100A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A1 fusion protein Ag8871
Full Name S100 calcium binding protein A1
Calculated Molecular Weight 94 aa, 11 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC014392
Gene Symbol S100A1
Gene ID (NCBI) 6271
RRIDAB_2183214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for S100A1 antibody 16027-1-APDownload protocol
IHC protocol for S100A1 antibody 16027-1-APDownload protocol
IF protocol for S100A1 antibody 16027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Biomaterials

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.

Authors - Xianzhen Dong
View Article
humanIHC

Mol Neurobiol

Anti-Apoptotic Effect of IGF1 on Schwann Exposed to Hyperglycemia is Mediated by Neuritin, a Novel Neurotrophic Factor.

Authors - Lingfei Yan
View Article
ratWB

Front Pharmacol

Reynoutrin Improves Ischemic Heart Failure in Rats Via Targeting S100A1.

Authors - Wenkai Yang
  • KD Validated
View Article
humanWB

Circ Heart Fail

S100A1 in Human Heart Failure: Lack of Recovery Following LVAD Support.

Authors - Mosi K Bennett
View Article
ratWB,IF

Mol Med Rep

S100A1 expression is increased in spinal cord injury and promotes inflammation, oxidative stress and apoptosis of PC12 cells induced by LPS via ERK signaling

Authors - Ye Bai
View Article
ratIF

Sci Adv

An injectable and adaptable hydrogen sulfide delivery system for modulating neuroregenerative microenvironment

Authors - Xianzhen Dong
View Article