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  • Featured Product
  • KD/KO Validated

S100A16 Polyclonal antibody

S100A16 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11456-1-AP

Synonyms

AAG13, DT1P1A7, Protein S100 A16, Protein S100 F, S100A16, S100F

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using S100A16 Polyclonal antibody (11456-1-AP)

    WB analysis of A431 using 11456-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using S100A16 Polyclonal antibody (11456-1-AP)

    WB analysis using 11456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using S100A16 Polyclonal antibody (11456-1-AP)

    WB analysis of MCF-7 using 11456-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11456-1-AP (S100A16 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of mouse lung using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human colon cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using S100A16 Polyclonal antibody (11456-1-AP)

    IHC staining of human lung cancer using 11456-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11456-1-AP (S100A16 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using S100A16 Polyclonal antibody (11456-1-AP)

    IF Staining of MCF-7 using 11456-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 11456-1-AP (S100A16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, DU 145 cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11456-1-AP targets S100A16 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A16 fusion protein Ag2023
Full Name S100 calcium binding protein A16
Calculated Molecular Weight 103 aa, 12 kDa
Observed Molecular Weight 9-12 kDa
GenBank Accession NumberBC019099
Gene Symbol S100A16
Gene ID (NCBI) 140576
RRIDAB_2269940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).

Protocols

Product Specific Protocols
WB protocol for S100A16 antibody 11456-1-APDownload protocol
IHC protocol for S100A16 antibody 11456-1-APDownload protocol
IF protocol for S100A16 antibody 11456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Int J Biol Sci

S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development

Authors - Yifang Hu
  • KD Validated
View Article
mouseWB,IF

Cell Mol Life Sci

S100A16 promotes acute kidney injury by activating HRD1-induced ubiquitination and degradation of GSK3β and CK1α.

Authors - Yifei Sun
  • KO Validated
View Article
humanWB,IF

FASEB J

Brain microvascular endothelial cell exosome-mediated S100A16 up-regulation confers small-cell lung cancer cell survival in brain.

Authors - Zhi-Hua Xu
View Article
humanWB,IP

Front Cell Dev Biol

Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and Epithelial-Mesenchymal Transition Process in Gastric Cancer

Authors - Xiaoying You
  • KD Validated
View Article
humanIHC, WB

Biomolecules

ADAMTS19 Suppresses Cell Migration and Invasion by Targeting S100A16 via the NF-κB Pathway in Human Gastric Cancer.

Authors - Yingming Jiang
View Article
humanWB,IHC,IP

PLoS One

S100A14 Interacts with S100A16 and Regulates Its Expression in Human Cancer Cells.

Authors - Sapkota Dipak D
View Article