S100P Polyclonal antibody

S100P Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11803-1-AP

Synonyms

MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue, human lung cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11803-1-AP targets S100P in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100P fusion protein Ag2379
Full Name S100 calcium binding protein P
Calculated Molecular Weight 95 aa, 11 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC006819
Gene Symbol S100P
Gene ID (NCBI) 6286
RRIDAB_2184577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

Protocols

Product Specific Protocols
WB protocol for S100P antibody 11803-1-APDownload protocol
IHC protocol for S100P antibody 11803-1-APDownload protocol
IF protocol for S100P antibody 11803-1-APDownload protocol
FC protocol for S100P antibody 11803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Am J Cancer Res

High expression of S100P is associated with unfavorable prognosis and tumor progression in patients with epithelial ovarian cancer.

Authors - Xiangyu Wang
humanIHC

Proteomics Clin Appl

Identification of distinctive protein expression patterns in colorectal adenoma.

Authors - F Lam Francis F
humanIHC

Diagn Pathol

Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion.

Authors - Jung Min Jung MJ
humanWB

Exp Ther Med

Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma.

Authors - Chengyu Wang
humanWB

Technol Cancer Res Treat

LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p

Authors - Zhaozhi Xia
humanIHC

Int J Mol Sci

Expression-Based Diagnosis, Treatment Selection, and Drug Development for Breast Cancer

Authors - Qing Ye