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S100P Monoclonal antibody

S100P Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G6C3

Cat no : 67485-1-Ig

Synonyms

MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P

Validation Data Gallery

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  • Western Blot (WB) analysis of BXPC-3 cells using S100P Monoclonal antibody (67485-1-Ig)

    WB analysis of BXPC-3 using 67485-1-Ig

    BXPC-3 cells were subjected to SDS PAGE followed by western blot with 67485-1-Ig (S100P antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human pancreas cancer using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human pancreas cancer using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human placenta using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human placenta using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human urothelial carcinoma using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using S100P Monoclonal antibody (67485-1-Ig)

    IHC staining of human urothelial carcinoma using 67485-1-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using S100P Monoclonal antibody (67485-1-Ig)

    IF Staining of HeLa using 67485-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100P antibody (67485-1-Ig, Clone: 1G6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inBXPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, human placenta tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67485-1-Ig targets S100P in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen S100P fusion protein Ag19115
Full Name S100 calcium binding protein P
Calculated Molecular Weight 95 aa, 11 kDa
Observed Molecular Weight 10 KDa
GenBank Accession NumberBC006819
Gene Symbol S100P
Gene ID (NCBI) 6286
RRIDAB_2882712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

Protocols

Product Specific Protocols
WB protocol for S100P antibody 67485-1-IgDownload protocol
IHC protocol for S100P antibody 67485-1-IgDownload protocol
IF protocol for S100P antibody 67485-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols