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S1PR5/EDG8 Polyclonal antibody

S1PR5/EDG8 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 13874-1-AP

Synonyms

Edg 8, EDG8, S1P receptor 5, S1P receptor Edg 8, S1P5, S1PR5, SPPR 1, SPPR 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using S1PR5/EDG8 Polyclonal antibody (13874-1-AP)

    WB analysis of mouse brain using 13874-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using S1PR5/EDG8 Polyclonal antibody (13874-1-AP)

    WB analysis of K-562 using 13874-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using S1PR5/EDG8 Polyclonal antibody (13874-1-AP)

    WB analysis of HeLa using 13874-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using S1PR5/EDG8 Polyclonal antibody (13874-1-AP)

    WB analysis of Jurkat using 13874-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using S1PR5/EDG8 Polyclonal antibody (13874-1-AP)

    WB analysis of HEK-293 using 13874-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using S1PR5/EDG8 Polyclonal antibody (13874-1-AP)

    FC experiment of SH-SY5Y using 13874-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug S1PR5/EDG8 antibody (13874-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13874-1-AP targets S1PR5/EDG8 in WB, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S1PR5/EDG8 fusion protein Ag4928
Full Name sphingosine-1-phosphate receptor 5
Calculated Molecular Weight 398 aa, 42 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC034703
Gene Symbol S1PR5
Gene ID (NCBI) 53637
RRIDAB_2182638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that elicits diverse physiological effect on most types of cells and tissues via five G protein-coupled receptors, S1PR1 to S1PR5. S1PRs differ in their tissue expression and cell lineage: S1PR1-3 are expressed throughout the immune, central nervous system (CNS), and cardiovascular system; S1PR4 is primarily expressed in lymphoid tissue; and S1PR5 is a predominant receptor in CNS white matter, spleen and natural killer cells (PMID: 21707490). S1PR5 (also known as EDG8) has been involved in the trafficking of NK cells and monocytes (PMID: 21911833; 23519784).

Protocols

Product Specific Protocols
WB protocol for S1PR5/EDG8 antibody 13874-1-APDownload protocol
FC protocol for S1PR5/EDG8 antibody 13874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.

Authors - Rie Matsushima-Nishiwaki
View Article
mouseWB

Drug Des Devel Ther

Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.

Authors - Dianna Liu
View Article