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SAFB2 Polyclonal antibody

SAFB2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11642-1-AP

Synonyms

KIAA0138, SAF B2, SAFB2, scaffold attachment factor B2

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SAFB2 Polyclonal antibody (11642-1-AP)

    WB analysis of MCF-7 using 11642-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using SAFB2 Polyclonal antibody (11642-1-AP)

    WB analysis of rat pancreas using 11642-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SAFB2 Polyclonal antibody (11642-1-AP)

    WB analysis of HeLa using 11642-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SAFB2 Polyclonal antibody (11642-1-AP)

    WB analysis of MCF-7 using 11642-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using SAFB2 Polyclonal antibody (11642-1-AP)

    WB analysis of BxPC-3 using 11642-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11642-1-AP (SAFB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SAFB2 Polyclonal antibody (11642-1-AP)

    IHC staining of human breast cancer using 11642-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11642-1-AP (SAFB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SAFB2 Polyclonal antibody (11642-1-AP)

    IF Staining of U2OS using 11642-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SAFB2 antibody (11642-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inMCF-7 cells, BxPC-3 cells, HeLa cells, rat pancreas tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11642-1-AP targets SAFB2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAFB2 fusion protein Ag2230
Full Name scaffold attachment factor B2
Calculated Molecular Weight528aa,57 kDa; 953aa,107 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC025279
Gene Symbol SAFB2
Gene ID (NCBI) 9667
RRIDAB_2301309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAFB2, also named as Scaffold attachment factor B2 or KIAA0138, is a 953 amino acid protein, which contains one SAP domain and one RRM (RNA recognition motif) domain. SAFB2 expressed at high levels in the CNS and at low levels in the liver. It also is expressed in a wide number of breast cancer cell lines. SAFB2 binds to scaffold/matrix attachment region (S/MAR) DNA and can function as an estrogen receptor corepressor and can also inhibit cell proliferation.

Protocols

Product Specific Protocols
WB protocol for SAFB2 antibody 11642-1-APDownload protocol
IHC protocol for SAFB2 antibody 11642-1-APDownload protocol
IF protocol for SAFB2 antibody 11642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Proteomics

Establishment of a Proteomic Profile Associated with Gonocyte and Spermatogonial Stem Cell Maturation and Differentiation in Neonatal Mice.

Authors - Bo Zheng
View Article