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SAPS3 Polyclonal antibody

SAPS3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16944-1-AP

Synonyms

C11orf23, KIAA1558, PP6R3, PPP6R3, SAP190, SAPL, SAPLa, SAPS domain family member 3, SAPS domain family, member 3, SAPS3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAPS3 Polyclonal antibody (16944-1-AP)

    WB analysis using 16944-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16944-1-AP (SAPS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SAPS3 Polyclonal antibody (16944-1-AP)

    WB analysis of mouse kidney using 16944-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16944-1-AP (SAPS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SAPS3 Polyclonal antibody (16944-1-AP)

    IHC staining of mouse kidney using 16944-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SAPS3 Polyclonal antibody (16944-1-AP)

    IHC staining of mouse kidney using 16944-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SAPS3 Polyclonal antibody (16944-1-AP)

    IHC staining of mouse liver using 16944-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SAPS3 Polyclonal antibody (16944-1-AP)

    IHC staining of mouse liver using 16944-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16944-1-AP (SAPS3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SAPS3 Polyclonal antibody (16944-1-AP)

    IF Staining of HepG2 using 16944-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16944-1-AP (SAPS3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, mouse kidney tissue
Positive IHC detected inmouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16944-1-AP targets SAPS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAPS3 fusion protein Ag10555
Full Name SAPS domain family, member 3
Calculated Molecular Weight 873 aa, 98 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC020953
Gene Symbol SAPS3
Gene ID (NCBI) 55291
RRIDAB_2183826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SAPS3 antibody 16944-1-APDownload protocol
IHC protocol for SAPS3 antibody 16944-1-APDownload protocol
IF protocol for SAPS3 antibody 16944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

A conserved acetylation switch enables pharmacological control of tubby-like protein stability.

Authors - Evan M Kerek
View Article
humanIP

Cell Death Dis

PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death

Authors - Guowei Wu
View Article