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SARS2 Polyclonal antibody

SARS2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17258-1-AP

Synonyms

mtSerRS, SARS, SARS2, SARSM, Serine tRNA ligase, SerRS, SerRSmt, SERS, Seryl tRNA(Ser/Sec) synthetase, SYS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis using 17258-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of HeLa using 17258-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of mouse liver using 17258-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SARS2 Polyclonal antibody (17258-1-AP)

    WB analysis of HepG2 using 17258-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS2 Polyclonal antibody (17258-1-AP)

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SARS2 Polyclonal antibody (17258-1-AP)

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SARS2 Polyclonal antibody (17258-1-AP)

    IF Staining of HepG2 using 17258-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17258-1-AP (SARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse liver tissue, HepG2 cells, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17258-1-AP targets SARS2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS2 fusion protein Ag10913
Full Name seryl-tRNA synthetase 2, mitochondrial
Calculated Molecular Weight 518 aa, 58 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC042912
Gene Symbol SARS2
Gene ID (NCBI) 54938
RRIDAB_2238863
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SARS2 antibody 17258-1-APDownload protocol
IHC protocol for SARS2 antibody 17258-1-APDownload protocol
IF protocol for SARS2 antibody 17258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.

Authors - Shasha Gong
View Article