SART3 Polyclonal antibody

SART3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18025-1-AP

Synonyms

DSAP1, hSART 3, KIAA0156, P100, p110, p110(nrb), RP11 13G14, SART 3, SART3, TIP110



Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human liver tissue, human spleen tissue, human kidney tissue, human lung cancer tissue, human ovary tissue, human placenta tissue, human skin tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18025-1-AP targets SART3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SART3 fusion protein Ag12590
Full Name squamous cell carcinoma antigen recognized by T cells 3
Calculated Molecular Weight 963 aa, 110 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC093784
Gene Symbol SART3
Gene ID (NCBI) 9733
RRIDAB_2254228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The process of mRNA splicing required assembly of pre-mRNA into a large, dynamic RNA-protein complex, in which 5 small nuclear RNAs and more than 50 protein components are incorporated into functional small nuclear ribonucleoproteins(snRNPs). SART3 interacts with U6 snRNA and functioned as a U6-specific recycling factor responsible for regeneration of base-paired U4/U6 snRNPs from post-spliceosomal free U4 and U6 snRNPs. Also it can interact and regulate Tat transactivation activity, and act as a cellular factor for HIV-1 gene expression and viral replication.

Protocols

Product Specific Protocols
WB protocol for SART3 antibody 18025-1-APDownload protocol
IHC protocol for SART3 antibody 18025-1-APDownload protocol
IP protocol for SART3 antibody 18025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIP

RNA

7SL RNA in Vertebrate Red Blood Cells.

Authors - Gaëlle J S Talhouarne
humanWB,IP

J Biol Chem

Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.

Authors - Zhe Chen

Mol Cell Biol

USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis.

Authors - Jaehyun Kim
humanIHC

Aging (Albany NY)

SART3, regulated by p53, is a biomarker for diagnosis, prognosis and immune infiltration in hepatocellular carcinoma

Authors - Jusen Nong