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SBNO1 Polyclonal antibody

SBNO1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25359-1-AP

Synonyms

Monocyte protein 3, MOP 3, MOP3, SBNO1, Sno

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SBNO1 Polyclonal antibody (25359-1-AP)

    WB analysis using 25359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SBNO1 Polyclonal antibody (25359-1-AP)

    WB analysis of Jurkat using 25359-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SBNO1 Polyclonal antibody (25359-1-AP)

    WB analysis of mouse brain using 25359-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using SBNO1 Polyclonal antibody (25359-1-AP)

    WB analysis of Raji using 25359-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SBNO1 Polyclonal antibody (25359-1-AP)

    WB analysis of HeLa using 25359-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using SBNO1 Polyclonal antibody (25359-1-AP)

    IP experiment of Jurkat using 25359-1-AP

    IP result of anti-SBNO1 (IP:25359-1-AP, 4ug; Detection:25359-1-AP 1:3000) with Jurkat cells lysate 1400 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SBNO1 Polyclonal antibody (25359-1-AP)

    IF Staining of HeLa using 25359-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 25359-1-AP (SBNO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, Raji cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25359-1-AP targets SBNO1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SBNO1 fusion protein Ag19005
Full Name strawberry notch homolog 1 (Drosophila)
Calculated Molecular Weight 1393 aa, 154 kDa
Observed Molecular Weight 145-154 kDa
GenBank Accession NumberBC133704
Gene Symbol SBNO1
Gene ID (NCBI) 55206
RRIDAB_2880042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SBNO1, also named as Monocyte protein 3 or MOP3, is a 1393 amino acid protein, which belongs to the SBNO family. SBNO1 play an important role in development of the vertebrate central nervous system. SBNO1 has four isoforms with MW 154 kDa and 80 kDa.

Protocols

Product Specific Protocols
WB protocol for SBNO1 antibody 25359-1-APDownload protocol
IF protocol for SBNO1 antibody 25359-1-APDownload protocol
IP protocol for SBNO1 antibody 25359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Asian J Androl

Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice.

Authors - Cong Shen
View Article