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SCGN Polyclonal antibody

SCGN Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 14919-1-AP

Synonyms

CALBL, SCGN, SECRET, Secretagogin, SEGN, setagin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using SCGN Polyclonal antibody (14919-1-AP)

    WB analysis of mouse pancreas using 14919-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SCGN Polyclonal antibody (14919-1-AP)

    WB analysis of rat brain using 14919-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using SCGN Polyclonal antibody (14919-1-AP)

    WB analysis of mouse colon using 14919-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using SCGN Polyclonal antibody (14919-1-AP)

    WB analysis of rat brain using 14919-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using SCGN Polyclonal antibody (14919-1-AP)

    WB analysis of mouse stomach using 14919-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using SCGN Polyclonal antibody (14919-1-AP)

    WB analysis of fetal human brain using 14919-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SCGN Polyclonal antibody (14919-1-AP)

    WB analysis of mouse liver using 14919-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14919-1-AP (SCGN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SCGN Polyclonal antibody (14919-1-AP)

    IP experiment of mouse brain using 14919-1-AP

    IP result of anti-SCGN (IP:14919-1-AP, 4ug; Detection:14919-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse pancreas tissue, fetal human brain tissue, mouse colon tissue, mouse liver tissue, mouse stomach tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14919-1-AP targets SCGN in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCGN fusion protein Ag6736
Full Name secretagogin, EF-hand calcium binding protein
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC000336
Gene Symbol SCGN
Gene ID (NCBI) 10590
RRIDAB_2183555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SCGN antibody 14919-1-APDownload protocol
IP protocol for SCGN antibody 14919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols