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SDSL Polyclonal antibody

SDSL Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16242-1-AP

Synonyms

L serine deaminase, L threonine dehydratase, SDH 2, SDS RS1, SDSL, Serine dehydratase 2, serine dehydratase like, TDH

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of HepG2 using 16242-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of human liver using 16242-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of human colon using 16242-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of mouse liver using 16242-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of mouse lung using 16242-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    WB analysis of human liver using 16242-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16242-1-AP (SDSL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SDSL Polyclonal antibody (16242-1-AP)

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16242-1-AP (SDSL antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SDSL Polyclonal antibody (16242-1-AP)

    IF Staining of HepG2 using 16242-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16242-1-AP (SDSL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human colon tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16242-1-AP targets SDSL in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDSL fusion protein Ag9114
Full Name serine dehydratase-like
Calculated Molecular Weight 329 aa, 35 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC009849
Gene Symbol SDSL
Gene ID (NCBI) 113675
RRIDAB_2254163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SDSL antibody 16242-1-APDownload protocol
IHC protocol for SDSL antibody 16242-1-APDownload protocol
IF protocol for SDSL antibody 16242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols