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  • Featured Product
  • KD/KO Validated

SEC16A Polyclonal antibody

SEC16A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20025-1-AP

Synonyms

KIAA0310, p250, RP11 413M3.10, SEC16, SEC16 homolog A, SEC16A, SEC16L

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SEC16A Polyclonal antibody (20025-1-AP)

    WB analysis of MCF-7 using 20025-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SEC16A Polyclonal antibody (20025-1-AP)

    WB analysis using 20025-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20025-1-AP (SEC16A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SEC16A Polyclonal antibody (20025-1-AP)

    IP experiment of HeLa using 20025-1-AP

    IP result of anti-SEC16A (IP:20025-1-AP, 4ug; Detection:20025-1-AP 1:2000) with HeLa cells lysate 840 ug.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC16A Polyclonal antibody (20025-1-AP)

    IHC staining of mouse pancreas using 20025-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC16A Polyclonal antibody (20025-1-AP)

    IHC staining of mouse pancreas using 20025-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20025-1-AP (SEC16A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SEC16A Polyclonal antibody (20025-1-AP)

    IF Staining of A431 using 20025-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SEC16A antibody (20025-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using SEC16A Polyclonal antibody (20025-1-AP)

    FC experiment of HEK-293 using 20025-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SEC16A (20025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20025-1-AP targets SEC16A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SEC16 homolog A (S. cerevisiae)
Calculated Molecular Weight 234 kDa
Observed Molecular Weight 250-300 kDa
GenBank Accession NumberNM_014866
Gene Symbol SEC16A
Gene ID (NCBI) 9919
RRIDAB_2878635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

Protocols

Product Specific Protocols
WB protocol for SEC16A antibody 20025-1-APDownload protocol
IHC protocol for SEC16A antibody 20025-1-APDownload protocol
IF protocol for SEC16A antibody 20025-1-APDownload protocol
IP protocol for SEC16A antibody 20025-1-APDownload protocol
FC protocol for SEC16A antibody 20025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Chem Biol

Functional dissection of the retrograde Shiga toxin trafficking inhibitor Retro-2.

Authors - Alison Forrester
  • KD Validated
View Article