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SEC24C Polyclonal antibody

SEC24C Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16073-1-AP

Synonyms

KIAA0079, SEC24 related protein C, SEC24C

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SEC24C Polyclonal antibody (16073-1-AP)

    WB analysis of HeLa using 16073-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SEC24C Polyclonal antibody (16073-1-AP)

    WB analysis of HeLa using 16073-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16073-1-AP (SEC24C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC24C Polyclonal antibody (16073-1-AP)

    IF Staining of HeLa using 16073-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16073-1-AP (SEC24C antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16073-1-AP targets SEC24C in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC24C fusion protein Ag9030
Full Name SEC24 family, member C (S. cerevisiae)
Calculated Molecular Weight 1094 aa, 118 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC018928
Gene Symbol SEC24C
Gene ID (NCBI) 9632
RRIDAB_2186121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

Protocols

Product Specific Protocols
WB protocol for SEC24C antibody 16073-1-APDownload protocol
IF protocol for SEC24C antibody 16073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols