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Septin 10 Polyclonal antibody

Septin 10 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12420-1-AP

Synonyms

SEPT10, septin 10, septin10

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using Septin 10 Polyclonal antibody (12420-1-AP)

    WB analysis of human kidney using 12420-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12420-1-AP (Septin 10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Septin 10 Polyclonal antibody (12420-1-AP)

    WB analysis of HeLa using 12420-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12420-1-AP (Septin 10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Septin 10 Polyclonal antibody (12420-1-AP)

    IHC staining of human pancreas cancer using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Septin 10 Polyclonal antibody (12420-1-AP)

    IF Staining of HepG2 using 12420-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman kidney tissue, HeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12420-1-AP targets Septin 10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 10 fusion protein Ag3071
Full Name septin 10
Calculated Molecular Weight 53 kDa, 57 kDa
Observed Molecular Weight 53-57 kDa
GenBank Accession NumberBC020502
Gene Symbol SEPT10
Gene ID (NCBI) 151011
RRIDAB_2186992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Septin 10 antibody 12420-1-APDownload protocol
IHC protocol for Septin 10 antibody 12420-1-APDownload protocol
IF protocol for Septin 10 antibody 12420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols