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SERCA2 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 13985-1-AP

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Con: 24 μg/150 μl

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Tested Applications

Applications:
WB, ELISA
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SERCA2 Antibody WB mouse heart tissue 13985-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 13985-1-AP(SERCA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue
Recommended dilution:
WB : 1:1000-1:6000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
SERCA2 fusion protein Ag5052
Full name:
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Calculated molecular weight:
1042aa,115 kDa
Observed molecular weight:
100 kDa
GenBank accession number:
BC035588
Gene ID (NCBI):
488
Gene symbol
ATP2A2
Synonyms
ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+) ATPase 2

Background

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types. This antibody can recognize all the above isoforms.


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