Alpha 1 Antitrypsin Polyclonal antibody

Name: Alpha 1 Antitrypsin Polyclonal antibody
Brand: Proteintech
SKU: 16382-1-AP

Alpha 1 Antitrypsin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16382-1-AP

Synonyms

A1A, A-1-Antitrypsin, A1AT, AAT, Alpha 1 antiproteinase, Alpha 1 antitrypsin, Alpha 1 protease inhibitor, PI, PI1, PRO2209, PRO2275, Serpin A1, SERPINA1, SPAAT, α1-antitrypsin

Validation Data Gallery

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AllWBIPIHCIFFC

WB analysis using 16382-1-AP
human saliva tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of human urine using 16382-1-AP
human urine sample were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16382-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 16382-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16382-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 16382-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16382-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP (Alpha-1-Antitrypsin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 16382-1-AP
IP result of anti-Alpha 1 Antitrypsin (IP:16382-1-AP, 4ug; Detection:16382-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug.
IHC staining of human liver cancer using 16382-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16382-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16382-1-PBS
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 16382-1-PBS
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 16382-1-PBS
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 16382-1-PBS
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 16382-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16382-1-AP (Alpha 1 Antitrypsin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16382-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha 1 Antitrypsin antibody (16382-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
FC experiment of HepG2 using 16382-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Alpha 1 Antitrypsin (16382-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 16382-1-AP

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Tested Applications

Positive WB detected in	human saliva tissue, human kidney tissue, human urine sample, mouse liver tissue, mouse skin tissue, mouse kidney tissue, mouse lung tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	mouse liver tissue, human liver cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)	IF : 1:200-1:800
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 16 publications below
IHC	See 3 publications below
IF	See 3 publications below

Product Information

16382-1-AP targets Alpha 1 Antitrypsin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Alpha 1 Antitrypsin fusion protein Ag9369
Full Name	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Calculated Molecular Weight	418 aa, 47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	BC015642
Gene Symbol	SERPINA1
Gene ID (NCBI)	5265
RRID	AB_10641185
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA1 is the gene for a protein called alpha-1-antitrypsin (AAT), which is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. AAT is a glycoprotein synthesized primarily by hepatocytes, with smaller amountssynthesized by intestinal epithelial cells, neutrophils, pulmonary alveolar cells and macrophages. AAT is the most abundant, endogenous serine protease inhibitor in blood circulation and it has been implicated in regulating vital fluid phase biological events such as blood coagulation, fibrinolysis, complement activation, apoptosis, reproduction, tumor progression and inflammatory response. The primary function of AAT is thought to be the inactivation of neutrophil elastase and other endogenous serine proteases. Defects in SERPINA1 can cause emphysema or liver disease.

Protocols

Product Specific Protocols
WB protocol for Alpha 1 Antitrypsin antibody 16382-1-AP	Download protocol
IHC protocol for Alpha 1 Antitrypsin antibody 16382-1-AP	Download protocol
IF protocol for Alpha 1 Antitrypsin antibody 16382-1-AP	Download protocol
IP protocol for Alpha 1 Antitrypsin antibody 16382-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection. Authors - Lukas Wettstein View Article
mouse	WB	Nat Commun The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation. Authors - Mehrpouya B Mobin View Article
human	IF	Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. Authors - Emna Ouni View Article
human	WB	J Nutr Biochem Characterization of dietary and herbal sourced natural compounds that modulate SEL1L-HRD1 ERAD activity and alleviate protein misfolding in the ER Authors - Jifeng Yang View Article
mouse	WB	Blood Adv Altered phenotype in LMAN1-deficient mice with low levels of residual LMAN1 expression. Authors - Lesley A Everett View Article
mouse	WB	Nutrients New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation. Authors - Po-An Hu View Article

Alpha 1 Antitrypsin Polyclonal antibody

Alpha 1 Antitrypsin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 16382-1-AP

WB analysis of human urine using 16382-1-AP

WB analysis of mouse lung using 16382-1-AP

WB analysis of human kidney using 16382-1-AP

WB analysis of mouse liver using 16382-1-AP

WB analysis of mouse skin using 16382-1-AP

WB analysis of mouse kidney using 16382-1-AP

IP experiment of mouse kidney using 16382-1-AP

IHC staining of human liver cancer using 16382-1-AP

IHC staining of mouse liver using 16382-1-AP

IHC staining of mouse liver using 16382-1-PBS

IHC staining of human hepatocirrhosis using 16382-1-PBS

IHC staining of human hepatocirrhosis using 16382-1-PBS

IHC staining of mouse liver using 16382-1-PBS

IHC staining of mouse kidney using 16382-1-AP

IF Staining of HepG2 using 16382-1-AP

FC experiment of HepG2 using 16382-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection.

The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation.

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Characterization of dietary and herbal sourced natural compounds that modulate SEL1L-HRD1 ERAD activity and alleviate protein misfolding in the ER

Altered phenotype in LMAN1-deficient mice with low levels of residual LMAN1 expression.

New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation.

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