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Alpha Antichymotrypsin Polyclonal antibody

Alpha Antichymotrypsin Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 12192-1-AP

Synonyms

A1 Antichymotrypsin, A-1-Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    WB analysis of human plasma using 12192-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    WB analysis of human colon using 12192-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    WB analysis of COLO 320 using 12192-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IP experiment of COLO 320 using 12192-1-AP

    IP result of anti-Alpha Antichymotrypsin (IP:12192-1-AP, 4ug; Detection:12192-1-AP 1:500) with COLO 320 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human tonsillitis using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human tonsillitis using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human liver using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human liver using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12192-1-AP (alpha 1 Antichymotrypsin antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human spleen using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human spleen using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human liver using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    IHC staining of human liver using 12192-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12192-1-AP (alpha 1 Antichymotrypsin antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HepG2 cells using Alpha Antichymotrypsin Polyclonal antibody (12192-1-AP)

    FC experiment of HepG2 using 12192-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Alpha Antichymotrypsin (12192-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inhuman blood tissue, COLO 320 cells, human colon tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman tonsillitis tissue, human liver tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12192-1-AP targets Alpha Antichymotrypsin in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Antichymotrypsin fusion protein Ag2830
Full Name serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3
Calculated Molecular Weight 448 aa, 48 kDa
Observed Molecular Weight 44-70 kDa
GenBank Accession NumberBC010530
Gene Symbol SERPINA3
Gene ID (NCBI) 12
RRIDAB_10344682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.

Protocols

Product Specific Protocols
WB protocol for Alpha Antichymotrypsin antibody 12192-1-APDownload protocol
IHC protocol for Alpha Antichymotrypsin antibody 12192-1-APDownload protocol
IP protocol for Alpha Antichymotrypsin antibody 12192-1-APDownload protocol
FC protocol for Alpha Antichymotrypsin antibody 12192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Acta Neuropathol

Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS

Authors - Amr Aly
View Article
humanWB

Front Immunol

Screening and identification of tissue-infiltrating immune cells and genes for patients with emphysema phenotype of COPD

Authors - Di Wang
View Article
humanWB,IHC

Br J Cancer

Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.

Authors - Yanxia Jin
View Article
mouseIHC

Front Med (Lausanne)

An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.

Authors - Songyan Gao
View Article

Proteomics Clin Appl

Serum protein profiling in diffuse large B-cell lymphoma.

Authors - Jacques Riby
View Article
human,ratWB

Food Funct

Sinensetin attenuates IL-1β-induced cartilage damage and ameliorates osteoarthritis by regulating SERPINA3

Authors - Zhendong Liu
View Article