Alpha Antichymotrypsin Monoclonal antibody
Alpha Antichymotrypsin Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
3F2F10
Cat no : 66078-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma, human colon tissue |
| Positive IHC detected in | human tonsillitis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 66078-1-Ig is Alpha Antichymotrypsin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Alpha Antichymotrypsin fusion protein Ag2830 |
| Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 |
| Calculated Molecular Weight | 448 aa, 48 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC010530 |
| Gene Symbol | SERPINA3 |
| Gene ID (NCBI) | 12 |
| RRID | AB_11182502 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson's disease (PD), Alzheimer's disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Antichymotrypsin antibody 66078-1-Ig | Download protocol |
| IHC protocol for Alpha Antichymotrypsin antibody 66078-1-Ig | Download protocol |
| IF protocol for Alpha Antichymotrypsin antibody 66078-1-Ig | Download protocol |
| FC protocol for Alpha Antichymotrypsin antibody 66078-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Cancer Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins. | ||
Breast Cancer Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells. | ||
Pharmaceutics Distribution of Monocarboxylate Transporters in Brain and Choroid Plexus Epithelium |
