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  • Featured Product
  • KD/KO Validated

SERPINB10 Polyclonal antibody

SERPINB10 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21719-1-AP

Synonyms

bomapin, Peptidase inhibitor 10, PI 10, PI10, Serpin B10, SERPINB10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPINB10 Polyclonal antibody (21719-1-AP)

    WB analysis using 21719-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using SERPINB10 Polyclonal antibody (21719-1-AP)

    WB analysis of HL-60 using 21719-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Sp2/0 cells using SERPINB10 Polyclonal antibody (21719-1-AP)

    WB analysis of Sp2/0 using 21719-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 21719-1-AP (SERPINB10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SERPINB10 Polyclonal antibody (21719-1-AP)

    IHC staining of human tonsillitis using 21719-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21719-1-AP (SERPINB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SERPINB10 Polyclonal antibody (21719-1-AP)

    IHC staining of human placenta using 21719-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SERPINB10 Polyclonal antibody (21719-1-AP)

    IHC staining of human placenta using 21719-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SERPINB10 Polyclonal antibody (21719-1-AP)

    IHC staining of human pancreas using 21719-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SERPINB10 Polyclonal antibody (21719-1-AP)

    IHC staining of human pancreas using 21719-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21719-1-AP (SERPINB10 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPINB10 Polyclonal antibody (21719-1-AP)

    IF Staining of HepG2 using 21719-1-AP

    Immunofluorescent analysis of HepG2 cells, using SERPINB10 antibody 21719-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA431 cells, HL-60 cells, Sp2/0 cells, human saliva
Positive IHC detected inhuman tonsillitis tissue, human pancreas tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21719-1-AP is SERPINB10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SERPINB10 fusion protein Ag16372
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 10
Calculated Molecular Weight 397 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC096217
Gene Symbol SERPINB10
Gene ID (NCBI) 5273
RRIDAB_10734592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SERPINB10 antibody 21719-1-APDownload protocol
IHC protocol for SERPINB10 antibody 21719-1-APDownload protocol
IF protocol for SERPINB10 antibody 21719-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Am J Physiol Lung Cell Mol Physiol

Epithelial SERPINB10, a novel marker of airway eosinophilia in asthma, contributes to allergic airway inflammation.

Authors - Yuqing Mo
  • KD Validated
View Article