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  • KD/KO Validated

HSP47 Polyclonal antibody

HSP47 Polyclonal Antibody for FC (Intra), IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10875-1-AP

Synonyms

47 kDa heat shock protein, AsTP3, CBP1, CBP2, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2



Tested Applications

Positive WB detected inHT-1080 cells, A2780 cells, A431 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman stomach cancer tissue, human colon tissue, human hepatocirrhosis tissue, human lung cancer tissue, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10875-1-AP targets HSP47 in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP47 fusion protein Ag1301
Full Name serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC014623
Gene Symbol SERPINH1
Gene ID (NCBI) 871
RRIDAB_2301500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

Protocols

Product Specific Protocols
WB protocol for HSP47 antibody 10875-1-APDownload protocol
IHC protocol for HSP47 antibody 10875-1-APDownload protocol
IF protocol for HSP47 antibody 10875-1-APDownload protocol
FC protocol for HSP47 antibody 10875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Protoc

Responsive and activable nanomedicines for remodeling the tumor microenvironment.

Authors - Yinlong Zhang
  • KD Validated
humanWB,IF,IHC

Nat Commun

Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.

Authors - Xuexiang Han
  • KD Validated
human,mouseWB,IHC

Cancer Res

RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.

Authors - Nicholas Rooney
humanIP

mSystems

Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.

Authors - Xiaoqing Liu
mouseWB

PLoS Genet

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

Authors - Masahiko Terajima
mouseWB

Sci Rep

Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes

Authors - Masahiko Terajima

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gohar (Verified Customer) (02-06-2020)

na

  • Applications: Immunohistochemistry,
FH

Gohar (Verified Customer) (02-06-2020)

na

  • Applications: Immunohistochemistry,
FH

Danielle (Verified Customer) (09-09-2019)

This antibody, as well as the other 5 purchased for our purposes, was sufficient and provided clear results for our experiment.

  • Applications: ChIP,