Neuroserpin Monoclonal antibody
Neuroserpin Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, pig, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1G4C6
Cat no : 66997-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue |
| Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF) | IF : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
66997-1-Ig targets Neuroserpin in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rat samples.
| Tested Reactivity | Human, pig, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Neuroserpin fusion protein Ag28636 |
| Full Name | serpin peptidase inhibitor, clade I (neuroserpin), member 1 |
| Calculated Molecular Weight | 410 aa, 47 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC018043 |
| Gene Symbol | SERPINI1 |
| Gene ID (NCBI) | 5274 |
| RRID | AB_2882314 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| IHC protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| IF protocol for Neuroserpin antibody 66997-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
