SF4 Polyclonal antibody

SF4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15614-1-AP

Synonyms

F23858, RBP, RNA binding protein RBP, SF4, splicing factor 4



Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15614-1-AP targets SF4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SF4 fusion protein Ag8006
Full Name splicing factor 4
Calculated Molecular Weight 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC002986
Gene Symbol SF4
Gene ID (NCBI) 57794
RRIDAB_10859818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SF4 antibody 15614-1-APDownload protocol
IHC protocol for SF4 antibody 15614-1-APDownload protocol
IF protocol for SF4 antibody 15614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols