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SFRS17A Polyclonal antibody

SFRS17A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13441-1-AP

Synonyms

721P, B lymphocyte antigen, CCDC133, CXYorf3, DXYS155E, Protein XE7, SFRS17A, XE7, XE7Y

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of HeLa using 13441-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of Jurkat using 13441-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of HEK-293T using 13441-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of human kidney using 13441-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    WB analysis of human heart using 13441-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    IHC staining of human pancreas cancer using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SFRS17A Polyclonal antibody (13441-1-AP)

    IHC staining of human pancreas cancer using 13441-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, human heart tissue, human kidney tissue, HEK-293T cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13441-1-AP targets SFRS17A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS17A fusion protein Ag4252
Full Name splicing factor, arginine/serine-rich 17A
Calculated Molecular Weight 695 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC028151
Gene Symbol SFRS17A
Gene ID (NCBI) 8227
RRIDAB_2184937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SFRS17A antibody 13441-1-APDownload protocol
IHC protocol for SFRS17A antibody 13441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols