Surfactant Protein A Polyclonal antibody

Surfactant Protein A Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 11850-1-AP

Synonyms

Alveolar proteinosis protein, COLEC4, Collectin 4, PSAP, PSP A, PSPA, SFTP1, SFTPA, SFTPA1, SFTPA1B, SFTPA2, SP A, SP A1, Surfactant Protein A, surfactant protein A1



Tested Applications

Positive IHC detected inhuman lung tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human brain tissue, human spleen tissue, human ovary tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11850-1-AP targets Surfactant Protein A in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Surfactant Protein A fusion protein Ag2417
Full Name surfactant protein A1
Calculated Molecular Weight 248 aa, 26 kDa
GenBank Accession NumberBC026229
Gene Symbol SFTPA1
Gene ID (NCBI) 653509
RRIDAB_2185360
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

Protocols

Product Specific Protocols
IHC protocol for Surfactant Protein A antibody 11850-1-APDownload protocol
FC protocol for Surfactant Protein A antibody 11850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Sci Total Environ

Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets

Authors - Xiaotong Wang
mouseWB

Int J Mol Sci

Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.

Authors - Guangying Yang
human,mouseIHC

Cancer Immunol Immunother

SFTPA1 is a potential prognostic biomarker correlated with immune cell infiltration and response to immunotherapy in lung adenocarcinoma.

Authors - Lu Yuan
mouseWB

Biochem Biophys Res Commun

Increased mortality from influenza infection in long-chain acyl-CoA dehydrogenase knockout mice.

Authors - Apurva Shinde
humanWB

Environ Toxicol

Silica nanoparticle exposure inhibits surfactant protein A and B in A549 cells through ROS-mediated JNK/c-Jun signaling pathway.

Authors - Xiaojing Yang
mouseWB

J Thorac Dis

Deletion of LCMR1 in alveolar type II cells induces lethal impairment of lung structure and function in adult mice

Authors - Yueming Wang