Beta Sarcoglycan Polyclonal antibody
Beta Sarcoglycan Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11936-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
11936-1-AP targets Beta Sarcoglycan in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Beta Sarcoglycan fusion protein Ag2533 |
| Full Name | sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) |
| Calculated Molecular Weight | 318 aa, 35 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC020709 |
| Gene Symbol | SGCB |
| Gene ID (NCBI) | 6443 |
| RRID | AB_10699870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SGCB is a 43-kD component of the dystrophin-glycoprotein complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Sarcoglycan antibody 11936-1-AP | Download protocol |
| IHC protocol for Beta Sarcoglycan antibody 11936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Cardioprotective effect of icariin against myocardial fibrosis and its molecular mechanism in diabetic cardiomyopathy based on network pharmacology: Role of ICA in DCM. | ||
Oncotarget Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Venkata (Verified Customer) (01-03-2022) | I have loaded 50 and 100ug of protein in each of the lanes and the samples are from mouse heart and skeletal muscle tissues. I don't see expected bands in all the samples and most of the bands are non specific.
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