SGCG Polyclonal antibody

SGCG Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18102-1-AP

Synonyms

35DAG, A4, DAGA4, DMDA, DMDA1, Gamma sarcoglycan, Gamma SG, LGMD2C, MAM, SCARMD2, SCG3, SGCG, TYPE



Tested Applications

Positive WB detected inmouse heart tissue, human skeletal muscle tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18102-1-AP targets SGCG in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGCG fusion protein Ag12402
Full Name sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC074777
Gene Symbol SGCG
Gene ID (NCBI) 6445
RRIDAB_2286186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SGCG antibody 18102-1-APDownload protocol
IHC protocol for SGCG antibody 18102-1-APDownload protocol
IP protocol for SGCG antibody 18102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF,IP

Skelet Muscle

Novel γ-sarcoglycan interactors in murine muscle membranes.

Authors - Tara C Smith
mouseIHC

PLoS One

Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy.

Authors - Alison M Blain
humanIF

PLoS One

Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies.

Authors - Narinder Janghra
humanIF

Hum Gene Ther Methods

Optimisation of internally deleted dystrophin constructs.

Authors - Mojgan Reza
mouseIHC

Mol Vis

Identification of HMX1 target genes: a predictive promoter model approach.

Authors - Boulling Arnaud A