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  • Featured Product
  • KD/KO Validated

SH3BP4 Polyclonal antibody

SH3BP4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 17691-1-AP

Synonyms

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SH3BP4 Polyclonal antibody (17691-1-AP)

    WB analysis using 17691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SH3BP4 Polyclonal antibody (17691-1-AP)

    WB analysis of HeLa using 17691-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SH3BP4 Polyclonal antibody (17691-1-AP)

    WB analysis of mouse lung using 17691-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using SH3BP4 Polyclonal antibody (17691-1-AP)

    IP experiment of mouse lung using 17691-1-AP

    IP result of anti-SH3BP4 (IP:17691-1-AP, 4ug; Detection:17691-1-AP 1:300) with mouse lung tissue lysate 3600ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH3BP4 Polyclonal antibody (17691-1-AP)

    IF Staining of HeLa using 17691-1-AP

    Immunofluorescent analysis of HeLa cells using 17691-1-AP (SH3BP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17691-1-AP targets SH3BP4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SH3BP4 fusion protein Ag11847
Full Name SH3-domain binding protein 4
Calculated Molecular Weight 963 aa, 108 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC057396
Gene Symbol SH3BP4
Gene ID (NCBI) 23677
RRIDAB_2189364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SH3BP4 antibody 17691-1-APDownload protocol
IF protocol for SH3BP4 antibody 17691-1-APDownload protocol
IP protocol for SH3BP4 antibody 17691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Mol Med

SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF.

Authors - Kyu-Han Kim
  • KD Validated
View Article
pigWB

J Appl Physiol (1985)

Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs.

Authors - Agus Suryawan
View Article

Nat Commun

Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival

Authors - Joanne Watson
View Article