Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 15422-1-AP

Synonyms

Bax interacting factor 1, Bif 1, Bif1, Endophilin B1, SH3GLB1



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15422-1-AP targets Bif-1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bif-1 fusion protein Ag7710
Full Name SH3-domain GRB2-like endophilin B1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC007455
Gene Symbol SH3GLB1
Gene ID (NCBI) 51100
RRIDAB_2254564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Bif-1 antibody 15422-1-APDownload protocol
IHC protocol for Bif-1 antibody 15422-1-APDownload protocol
IF protocol for Bif-1 antibody 15422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

Eur J Pharmacol

SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.

Authors - Anbo Gao
humanWB

J Exp Clin Cancer Res

SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.

Authors - Chia-Hung Chien
mouseWB

Oncotarget

Autophagy-related gene expression is an independent prognostic indicator of glioma.

Authors - Huixue Zhang
humanWB,ChIP

BMC Cancer

Role of SH3GLB1 in the regulation of CD133 expression in GBM cells

Authors - Chia-Hung Chien