SHBG Polyclonal antibody

SHBG Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18200-1-AP

Synonyms

ABP, Androgen binding protein, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG



Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inmouse testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

18200-1-AP targets SHBG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitygoat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHBG fusion protein Ag12683
Full Name sex hormone-binding globulin
Calculated Molecular Weight 402 aa, 44 kDa
Observed Molecular Weight 42-44 kDa
GenBank Accession NumberBC101785
Gene Symbol SHBG
Gene ID (NCBI) 6462
RRIDAB_2878517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

Protocols

Product Specific Protocols
WB protocol for SHBG antibody 18200-1-APDownload protocol
IHC protocol for SHBG antibody 18200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
goatWB

Mol Cell Endocrinol

FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells.

Authors - Huaming Xi
goatWB

Biol Reprod

Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season.

Authors - Huaming Xi
WB

Theriogenology

FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway

Authors - Huaming Xi