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SHOX Polyclonal antibody

SHOX Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 25117-1-AP

Synonyms

GCFX, PHOG, short stature homeobox, Short stature homeobox protein, SHOX, SHOXY, SS

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SHOX Polyclonal antibody (25117-1-AP)

    WB analysis of mouse brain using 25117-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25117-1-AP (SHOX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using SHOX Polyclonal antibody (25117-1-AP)

    WB analysis of fetal human brain using 25117-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25117-1-AP (SHOX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25117-1-AP is SHOX Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHOX fusion protein Ag18930
Full Name short stature homeobox
Calculated Molecular Weight 292 aa, 32 kDa
Observed Molecular Weight 25-32 kDa
GenBank Accession NumberBC148783
Gene Symbol SHOX
Gene ID (NCBI) 6473
RRIDAB_2879905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Short stature homeobox protein (SHOX), also named as GCFX, is a 292 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. SHOX. SHOX controls fundamental aspects of growth and development. SHOX undergoes splicing resulting in two isoforms, SHOXA and SHOXB. SHOXA is a 32 kDa protein and is expressed in skeletal muscle, placental, pancreas, heart and bone marrow fibroblast and, to a lesser extent, in kidney and lung. SHOXB is 25 kDa protein and is highly expressed in osteogenic cells, but not expressed in brain, kidney, liver or lung.

Protocols

Product Specific Protocols
WB protocol for SHOX antibody 25117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols