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SIPA1L1 Polyclonal antibody

SIPA1L1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25086-1-AP

Synonyms

E6 targeted protein 1, E6TP1, KIAA0440, SIPA1 like protein 1, SIPA1L1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIPA1L1 Polyclonal antibody (25086-1-AP)

    WB analysis using 25086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SIPA1L1 Polyclonal antibody (25086-1-AP)

    WB analysis of HeLa using 25086-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IP experiment of mouse brain using 25086-1-AP

    IP result of anti-SIPA1L1 (IP:25086-1-AP, 4ug; Detection:25086-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human brain using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human brain using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human pancreas using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human pancreas using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25086-1-AP targets SIPA1L1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIPA1L1 fusion protein Ag18900
Full Name signal-induced proliferation-associated 1 like 1
Calculated Molecular Weight 1803 aa, 200 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC146777
Gene Symbol SIPA1L1
Gene ID (NCBI) 26037
RRIDAB_2714023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SIPA1L1 antibody 25086-1-APDownload protocol
IHC protocol for SIPA1L1 antibody 25086-1-APDownload protocol
IP protocol for SIPA1L1 antibody 25086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Neuron

Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.

Authors - Sophie Laguesse
View Article