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  • Featured Product
  • KD/KO Validated

SIX1 Polyclonal antibody

SIX1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10709-1-AP

Synonyms

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of A2780 using 10709-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of SKOV-3 using 10709-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of HepG2 using 10709-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of L02 using 10709-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of L02 using 10709-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of SKOV-3 using 10709-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of HEK-293 using 10709-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of HEK-293 using 10709-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of HepG2 using 10709-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of L02 using 10709-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of HEK-293 using 10709-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of PC-3 using 10709-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SIX1 Polyclonal antibody (10709-1-AP)

    WB analysis of PC-3 using 10709-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SIX1 Polyclonal antibody (10709-1-AP)

    IP experiment of HEK-293 using 10709-1-AP

    IP result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

  • Immunofluorescence (IF) / fluorescent staining of epithelial tumor cells using SIX1 Polyclonal antibody (10709-1-AP)

    IF Staining of epithelial tumor cells using 10709-1-AP

    IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT.

Tested Applications

Positive WB detected inA2780 cells, HEK-293 cells, HepG2 cells, L02 cells, PC-3 cells, SKOV-3 cells
Positive IP detected inHEK-293 cells
Positive IF detected inepithelial tumor cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10709-1-AP targets SIX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIX1 fusion protein Ag1044
Full Name SIX homeobox 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberBC008874
Gene Symbol SIX1
Gene ID (NCBI) 6495
RRIDAB_2189077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

Protocols

Product Specific Protocols
WB protocol for SIX1 antibody 10709-1-APDownload protocol
IP protocol for SIX1 antibody 10709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
  • KO Validated
View Article
mouseWB

Nat Commun

Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology.

Authors - Nan Zhang
View Article
mouse

PLoS Biol

Dynamic enhancers control skeletal muscle identity and reprogramming.

Authors - Krithika Ramachandran
View Article
humanWB

Theranostics

O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.

Authors - Yi Chu
  • KD Validated
View Article
humanIF

Oncogene

The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes.

Authors - I Adrados
View Article
humanWB

Mol Ther Oncolytics

miR-489-3p/SIX1 Axis Regulates Melanoma Proliferation and Glycolytic Potential.

Authors - Xuhui Yang
  • KD Validated
View Article