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SIX1 Monoclonal antibody

SIX1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D7D1

Cat no : 67741-1-Ig

Synonyms

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIX1 Monoclonal antibody (67741-1-Ig)

    WB analysis using 67741-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SIX1 Monoclonal antibody (67741-1-Ig)

    WB analysis using 67741-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SIX1 Monoclonal antibody (67741-1-Ig)

    WB analysis using 67741-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SIX1 Monoclonal antibody (67741-1-Ig)

    IF Staining of A431 using 67741-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inL02 cells, HeLa cells, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67741-1-Ig targets SIX1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Reactivity Human, mouse, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIX1 fusion protein Ag28570
Full Name SIX homeobox 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC008874
Gene Symbol SIX1
Gene ID (NCBI) 6495
RRIDAB_2918510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

Protocols

Product Specific Protocols
WB protocol for SIX1 antibody 67741-1-IgDownload protocol
IF protocol for SIX1 antibody 67741-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols