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SIX5 Polyclonal antibody

SIX5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22938-1-AP

Synonyms

BOR2, DMAHP, Homeobox protein SIX5, Sine oculis homeobox homolog 5, SIX homeobox 5, SIX5

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using SIX5 Polyclonal antibody (22938-1-AP)

    WB analysis of Y79 using 22938-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 22938-1-AP (SIX5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SIX5 Polyclonal antibody (22938-1-AP)

    WB analysis of HeLa using 22938-1-AP

    WB result of SIX5 antibody (22938-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIX5 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of Y79 cells using SIX5 Polyclonal antibody (22938-1-AP)

    IP experiment of Y79 using 22938-1-AP

    IP result of anti-SIX5 (IP:22938-1-AP, 4ug; Detection:22938-1-AP 1:300) with Y79 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SIX5 Polyclonal antibody (22938-1-AP)

    IHC staining of human kidney using 22938-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22938-1-AP (SIX5 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inY79 cells, HeLa cells
Positive IP detected inY79 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

22938-1-AP targets SIX5 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIX5 fusion protein Ag18993
Full Name SIX homeobox 5
Calculated Molecular Weight 739 aa, 75 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC156983
Gene Symbol SIX5
Gene ID (NCBI) 147912
RRIDAB_2879186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SIX5 antibody 22938-1-APDownload protocol
IHC protocol for SIX5 antibody 22938-1-APDownload protocol
IP protocol for SIX5 antibody 22938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

iScience

RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis

Authors - Hannah J Wiedner
View Article