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SLAIN1 Polyclonal antibody

SLAIN1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 22123-1-AP

Synonyms

C13orf32, SLAIN motif family, member 1, SLAIN1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SLAIN1 Polyclonal antibody (22123-1-AP)

    WB analysis of HeLa using 22123-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22123-1-AP (SLAIN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SLAIN1 Polyclonal antibody (22123-1-AP)

    WB analysis of mouse brain using 22123-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22123-1-AP (SLAIN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLAIN1 Polyclonal antibody (22123-1-AP)

    WB analysis of HEK-293 using 22123-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22123-1-AP (SLAIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SLAIN1 Polyclonal antibody (22123-1-AP)

    IF Staining of HeLa using 22123-1-AP

    Immunofluorescent analysis of HeLa cells using 22123-1-AP (SLAIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SLAIN1 Polyclonal antibody (22123-1-AP)

    IF Staining of HEK-293 using 22123-1-AP

    Immunofluorescent analysis of HEK-293 cells using 22123-1-AP (SLAIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue
Positive IF detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22123-1-AP is SLAIN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLAIN1 fusion protein Ag4973
Full Name SLAIN motif family, member 1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 65-70 kDa, 34-40 kDa
GenBank Accession NumberBC045177
Gene Symbol SLAIN1
Gene ID (NCBI) 122060
RRIDAB_2878999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLAIN1 antibody 22123-1-APDownload protocol
IF protocol for SLAIN1 antibody 22123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Sci Rep

Identifying potential biomarkers of idiopathic pulmonary fibrosis through machine learning analysis

Authors - Zenan Wu
View Article