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SLAMF6 Polyclonal antibody

SLAMF6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22711-1-AP

Synonyms

Activating NK receptor, CD352, KALI, KALIb, Ly108, NK T B antigen, NTB A, NTBA, SF2000, SLAM family member 6, SLAMF6

Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using SLAMF6 Polyclonal antibody (22711-1-AP)

    WB analysis of Daudi using 22711-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 22711-1-AP (SLAMF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human colon cancer using 22711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human tonsillitis using 22711-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human colon cancer using 22711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human tonsillitis using 22711-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inDaudi cells
Positive IHC detected inhuman colon cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22711-1-AP targets SLAMF6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLAMF6 fusion protein Ag18517
Full Name SLAM family member 6
Calculated Molecular Weight 332 aa, 37 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC114495
Gene Symbol SLAMF6
Gene ID (NCBI) 114836
RRIDAB_2879154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobin (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in human only), double positive thymocytes, eosinophils and neutrophils (mouse only) (PMID:11862385) .

Protocols

Product Specific Protocols
WB protocol for SLAMF6 antibody 22711-1-APDownload protocol
IHC protocol for SLAMF6 antibody 22711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols