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SLC22A7 Polyclonal antibody

SLC22A7 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26796-1-AP

Synonyms

hOAT2, NLT, Novel liver transporter, OAT2, Organic anion transporter 2, SLC22A7

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using SLC22A7 Polyclonal antibody (26796-1-AP)

    WB analysis of mouse kidney using 26796-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using SLC22A7 Polyclonal antibody (26796-1-AP)

    WB analysis of rat liver using 26796-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC22A7 Polyclonal antibody (26796-1-AP)

    WB analysis of HEK-293 using 26796-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SLC22A7 Polyclonal antibody (26796-1-AP)

    IP experiment of mouse liver using 26796-1-AP

    IP result of anti-SLC22A7 (IP:26796-1-AP, 4ug; Detection:26796-1-AP 1:1000) with mouse liver tissue lysate 5200ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC22A7 Polyclonal antibody (26796-1-AP)

    IHC staining of human liver using 26796-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SLC22A7 Polyclonal antibody (26796-1-AP)

    IHC staining of human liver using 26796-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SLC22A7 Polyclonal antibody (26796-1-AP)

    IHC staining of mouse liver using 26796-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

The immunogen of 26796-1-AP is SLC22A7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC22A7 fusion protein Ag25217
Full Name solute carrier family 22 (organic anion transporter), member 7
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC033805
Gene Symbol SLC22A7
Gene ID (NCBI) 10864
RRIDAB_2880639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC22A7 antibody 26796-1-APDownload protocol
IHC protocol for SLC22A7 antibody 26796-1-APDownload protocol
IP protocol for SLC22A7 antibody 26796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Upregulation of histone acetylation reverses organic anion transporter 2 repression and enhances 5-fluorouracil sensitivity in hepatocellular carcinoma.

Authors - Yingying Wang
View Article
mouseWB

Nutrients

Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.

Authors - Karolin Weitkunat
View Article
humanWB

Drug Metab Pharmacokinet

Inhibiting uptake activity of organic anion transporter 2 by bile acids.

Authors - Yu Wang
View Article
mouseWB

Xenobiotica

Changes in the expression of drug-metabolising enzymes and drug transporters in mice with collagen antibody-induced arthritis

Authors - Chieri Fujino
View Article
mouse

Am J Physiol Renal Physiol

Tubular Dysfunction Impairs Renal Excretion of Pseudouridine in Diabetic Kidney Disease

Authors - Anna V Mathew
View Article