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- KD/KO Validated
SLC27A3 Polyclonal antibody
SLC27A3 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12943-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, mouse testis tissue, MCF-7 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human skin cancer tissue, human breast cancer tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
Product Information
12943-1-AP targets SLC27A3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SLC27A3 fusion protein Ag4015 |
| Full Name | solute carrier family 27 (fatty acid transporter), member 3 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 70-79 kDa |
| GenBank Accession Number | BC029792 |
| Gene Symbol | SLC27A3 |
| Gene ID (NCBI) | 11000 |
| RRID | AB_2286200 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC27A3 antibody 12943-1-AP | Download protocol |
| IHC protocol for SLC27A3 antibody 12943-1-AP | Download protocol |
| IP protocol for SLC27A3 antibody 12943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer. | ||
Eur J Pharmacol A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate | ||
Front Immunol Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. | ||
Mol Reprod Dev Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport. | ||
Int J Mol Sci Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development |
