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SMEK2 Polyclonal antibody

SMEK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20348-1-AP

Synonyms

FLFL2, KIAA1387, PP4R3B, PPP4R3B, PSY2, SMEK homolog 2, SMEK2, smk1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SMEK2 Polyclonal antibody (20348-1-AP)

    WB analysis of HEK-293 using 20348-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using SMEK2 Polyclonal antibody (20348-1-AP)

    WB analysis of A2780 using 20348-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SMEK2 Polyclonal antibody (20348-1-AP)

    WB analysis of HeLa using 20348-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SMEK2 Polyclonal antibody (20348-1-AP)

    WB analysis of MCF-7 using 20348-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SMEK2 Polyclonal antibody (20348-1-AP)

    IHC staining of human cervical cancer using 20348-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20348-1-AP (SMEK2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SMEK2 Polyclonal antibody (20348-1-AP)

    IHC staining of human cervical cancer using 20348-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20348-1-AP (SMEK2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SMEK2 Polyclonal antibody (20348-1-AP)

    IF Staining of HEK-293 using 20348-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 20348-1-AP (SMEK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A2780 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20348-1-AP is SMEK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMEK2 fusion protein Ag14156
Full Name SMEK homolog 2, suppressor of mek1 (Dictyostelium)
Calculated Molecular Weight 849 aa, 97 kDa
Observed Molecular Weight 94-97 kDa, 87 kDa
GenBank Accession NumberBC060855
Gene Symbol SMEK2
Gene ID (NCBI) 57223
RRIDAB_10694150
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SMEK2 antibody 20348-1-APDownload protocol
IHC protocol for SMEK2 antibody 20348-1-APDownload protocol
IF protocol for SMEK2 antibody 20348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K.

Authors - Shujuan Tong
View Article