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SMYD4 Monoclonal antibody

SMYD4 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2F4B7

Cat no : 66874-1-Ig

Synonyms

KIAA1936, SMYD4, ZMYND21

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SMYD4 Monoclonal antibody (66874-1-Ig)

    WB analysis of MCF-7 using 66874-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66874-1-Ig (SMYD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66874-1-Ig targets SMYD4 in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMYD4 fusion protein Ag11552
Full Name SET and MYND domain containing 4
Calculated Molecular Weight 804 aa, 89 kDa
Observed Molecular Weight 85-89 kDa
GenBank Accession NumberBC035077
Gene Symbol SMYD4
Gene ID (NCBI) 114826
RRIDAB_2882208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMYD4, also named as SET and MYND domain-containing protein 4, is a 804 amino acid protein, which contains 1 MYND-type zinc finger and 1 SET domain. SMYD4 belongs to the class V-like SAM-binding methyltransferase superfamily. SMYD4, as a potential tumor suppressor, plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of Pdgfr-alpha, and could be a novel target for improving treatment of breast cancer. SMYD4 is a muscle-specific transcriptional modulator involved in development.

Protocols

Product Specific Protocols
WB protocol for SMYD4 antibody 66874-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols